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Run: Dental Sterilizer Biological (Spore) Monitoring Log

Weekly dental sterilizer spore testing log for documenting biological indicator results, load release decisions, and corrective actions after failed or posit...

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Inspection Details

Inspection date and time recorded critical (required)

Sterilizer identifier documented critical (required)

Record the autoclave or sterilizer ID, location, or asset tag used for this test.

Operator or inspector name recorded critical (required)

Weekly biological indicator test performed critical (required)

Confirm that the sterilizer was tested this week with a biological indicator/spore test.

Yes No N/A

Biological Indicator Test Details

Biological indicator lot or control number recorded critical (required)

Test package or indicator placed in the most challenging load location critical (required)

Verify the BI was placed according to the sterilizer manufacturer's instructions and facility SOP.

Yes No N/A

Sterilization cycle parameters recorded critical (required)

Document cycle type, temperature, exposure time, and any other required cycle parameters.

Incubation start and read time recorded critical (required)

Record when the biological indicator was placed into incubation and when the result was read.

Control indicator result recorded critical (required)

Record the control result for the biological indicator test.

Test Result Review

Biological indicator result critical (required)

Record the final spore test result for the sterilizer cycle.

Result reviewed against acceptance criteria critical (required)

Confirm the result was compared to the manufacturer's instructions and facility acceptance criteria.

Yes No N/A

Load released only after acceptable BI result critical (required)

Confirm no instruments from the tested load were released before a passing biological indicator result, if applicable to the process.

Yes No N/A

Additional affected loads identified critical (required)

Indicate whether any other loads processed since the last acceptable BI test may be affected.

Yes No N/A

Corrective Action for Failed or Positive Tests

Failed or positive result investigated immediately critical (required)

Confirm the positive/failed BI result triggered immediate investigation per facility procedure.

Yes No N/A

Sterilizer removed from service pending resolution critical (required)

Confirm the sterilizer was taken out of service until the cause was identified and corrected, if required by policy.

Yes No N/A

Affected instruments quarantined or reprocessed critical (required)

Confirm any potentially affected instruments were quarantined, recalled, or reprocessed as appropriate.

Yes No N/A

Root cause documented

Describe the suspected cause of the failed test, such as operator error, packaging issue, sterilizer malfunction, or loading error.

Corrective action completed and verified critical (required)

Document the corrective action taken, the person responsible, and verification that the sterilizer returned to acceptable performance.

Documentation and Sign-off

Supporting documentation attached

Attach BI strip/photo, incubator printout, sterilizer cycle printout, and any corrective action records as applicable.

Yes No N/A

Photo evidence (optional)

Supervisor or infection control review completed critical (required)

Confirm review by the designated supervisor, infection control lead, or responsible manager.

Yes No N/A

Inspector signature critical (required)

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